Abstract:Plant biotechnology provide a new way to breed insect resistant rice. Cowpea trypsin inhibitor(cpti) gene is an insecticidal gene with wide range spectrum of insect-resistance. A novel strategy was employed in this research by modifying cpti gene in vitro. The translation product of modified cpti gene(sck gene) could be sorted in endoplasmic reticulm(ER) and protein body derived from ER and therefore increase foreign insecticidal protein stability in transgenic plant cell. Experiments result shows that both the accumulation of CpTI protein and insect resistant ability of sck transgenic plants increased greatly. Up to now, several types of transgenic rice line, include the excellent restorers Minghui81 and Minghui86, were attained and the hybridized combination have been made. Performance trial had shown that the transgenic rice had excellent insectcidal attribute and retain their eminent agrinomic trait.