Abstract:With the outbreak of avian flu, tuberculosis, Ebola and other epidemics, more and more global medical workers and researchers are engaging in dangerous pathogen related activities in recent years. These activities include diagnostics, testing, research, development, production and teaching. All of these activities must be carried out in the high-level biosafety laboratories. We compared the development status and characteristics of the international and domestic high-level biosafety laboratory and analyzed the problems of high-level biosafety laboratory system in China, such as the general layout, the fund input,the building of the management and the supporting system. We made several suggestions that China should promote the building of its high-level biosafety laboratory system to support the emerging infectious disease response,such us improving the overall layout of the laboratory, increasing investment, strengthening the management and the supporting system, strengthening information and resource sharing.