Abstract:The long-term ecological monitoring and research revealed the structure, function, and maintenance mechanism of forest biodiversity and ecosystem in the north subtropical zone, clarified the impact process of disturbance on biodiversity and ecosystem, condensed the global outstanding value of Shennongjia Natural Heritage, and constructed the “corridor stepping stone key habitat” protection system to enhance the ecosystem authenticity of the nature reserve. The key technology system and application mode of biodiversity protection and ecological restoration in national key ecological function areas were proposed. Long term ecological research provides basic theoretical and technical support for forest ecosystem research, biodiversity protection, ecological construction and sustainable economic and social development in north subtropical China.