Abstract:Green manufacturing is a modern manufacturing mode in the consideration of both environmental impact and efficiency of resource utilization, it represents a significant component of the “new productive force”. Centered on cell factories, green biomanufacturing leverages renewable resources to efficiently produce products that conform to the concepts of low-carbon, green, and sustainable development. The design of high-performance cell factories is pivotal for achieving efficient green biomanufacturing. Nevertheless, traditional cell factory design processes are complex, time-consuming, and labor-intensive. With advancements in artificial intelligence, digital-assisted cell factory design has emerged as a solution to save time and reduce costs. By integrating technologies such as database construction and updating, digital metabolic flux control design, digital retrosynthetic pathway design for target molecules, digital genetic circuit design, and fermentation process digital twin modeling, the full lifecycle digital design of cell factories can be achieved. This approach enables the rapid construction of high-performance cell factories, empowering green biomanufacturing.