Abstract:One main objective of metabolic engineering is to rewire the metabolic network for efficient production of biochemicals. Due to the complexity of cellular metabolic networks, it is often not straightforward to identify the proper modification targets from thousands of metabolic genes. Therefore, a time-consuming trial & error process is often required for the successful development. Aided by computational modeling of large-scale metabolic networks, one can design optimal pathways for synthesis of objective products, reducing the uncertainty of development and thus accelerating the strain construction process. In this short text, we give brief introduction to metabolic engineering design methods from two aspects:how to modify an organism to produce new chemicals with higher yields, and how to improve the cellular adaptation to the changing process conditions by integrating gene circuits. The computer aided design approach together with automated genome edition technologies, will greatly enhance the efficiency of the construction of artificial cell factories.