Abstract:The unique and extreme ecological environment of cryosphere acts as a gigantic reservoir of microorganisms, which preserves precious records of the diversity and evolutionary history of ancient microbes. Climate change and human activities have made significant impacts on the ecological system. Global warming causes melting of the cryosphere that remained frozen for thousands of years and releases microorganisms, some of them are recovered to life and constitute a potential threat to the ecological environment and human health. It is urgent to combine multidisciplinary research forces to investigate the microbial ecology of cryosphere, analyze the microbe pathogenesis and transmission mechanisms, as well as evaluate the biosecurity risks caused by the reboot of dormant microorganisms.