(1.Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;2.Key Laboratory of Industrial Biocatalysts, Ministry of Education, Beijing 100084, China;3.TMAXTREE Biotechnology Co. Ltd., Luoyang 471023, China;4.Beijing Key Laboratory of Biomass Waste Resource Utilization, College of Biochemical Engineering, Beijing Union University, Beijing 100023, China;5.Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China;6.Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China)
Abstract:Engineering cells are a core component of green bio-manufacturing. The efficient testing and acquisition of engineering cells with the desired phenotype is a critical factor in the successful implementation of bio-manufacturing. In recent years, based on microfluidics technologies, non-culture type single-cell phenotype high-throughput test equipment and culture type phenotype highthroughput test equipment for the growth and metabolism of single cell in a bioreactor have been developed such as microdroplet and microchamber, realizing high-throughput, automated, miniaturized, and integrated testing of the phenotypes of engineering cells and providing a powerful tool for the breeding of engineering cells. This review systematically compiles the research progress of nonculture type single-cell phenotype testing technologies and equipment based on fluorescence signals, Raman signals, image signals, and magnetic signals, as well as the droplet and microchamber culture type single-cell test technologies and equipment based on picoliter, nanoliter, and microliter multi-scale microbioreactors, and explores the development trend of the creation of high-throughput testing instruments for engineering cells, providing a reference for the research of green bio-manufacturing engineering cells testing technologies.
引用文本:
李爽,郭肖杰,陈海博,陈思思,笪鑫,李峥辉,刘芹秀,王怡,邢新会,张翀.基于微流控技术的工程细胞高通量表型测试技术与装备研究进展[J].中国科学院院刊,2025,40(1):91-106.
LI Shuang,GUO Xiaojie,CHEN Haibo,CHEN Sisi,DA Xin,LI Zhenghui,LIU Qinxiu,WANG yi,XING Xinhui,ZHANG Chong.Research progress in high-throughput phenotype testing technology and equipment for engineering cells based on microfluidics[J].Bulletin of Chinese Academy of Sciences,2025,40(1):91-106.